Catalytic Protein Discovery & Engineering
We discover and redesign catalytic proteins to expand the chemical capabilities of biology. Our work spans enzymatic fluorination, carbon capture enzymes, and new-to-nature chemistry, with an emphasis on mechanistic understanding and rational redesign
Fluorinase Discovery & Engineering
We identify and characterize new fluorinases to expand biological fluorination chemistry. Through structure-guided mutagenesis and kinetic analysis, we define active-site and long range determinants governing substrate scope and catalytic efficiency. These studies enable redesign of enzymatic C–F bond formation for radiochemical applications.
Defluorinase Engineering for Detoxification
To address the environmental persistence of fluorinated compounds, we investigate enzymatic strategies for C–F bond cleavage. By mapping catalytic mechanisms and engineering active-site architecture, we aim to develop biocatalytic platforms for fluorinated compound transformation.
Carbonic Anhydrase: Mechanism & Environmental Applications
Carbonic anhydrase provides a model system for understanding zinc-dependent catalysis and proton transfer networks. We combine mechanistic enzymology with protein engineering to explore enhanced CO₂ capture and environmental applications, while dissecting structure–function relationships in metalloenzymes.